CHO Chemically Defined Media
Single Cell Cloning Assay
CH150 medium (CD medium)
For CHO cells "CHO-K1, CHO-S, DG44, DXB11, etc."
CH150 medium is a chemically defined medium / serum-free medium optimized for the single-cell cloning of Chinese hamster ovary (CHO) cells, such as CHO-K1, CHO-S, DG44, and DXB11 cells. The medium contains no proteins, hydrolysates, or human/animal-derived components. All components have a known chemical structure.
Single-cell cloning in CHO cells is an important step in producing homogenous recombinant protein and allows homogeneity of a selected cell line. In general, serum-free medium results in very low cell cloning efficientry, and also typically fails to establish a stable cell clone producing recombinant protein. CH150 medium has enabled a single-cell cloninng for CHO cells.
Figure 1）Single cell cloning assay of CHO cells using CH150 medium.
Suspension CHO cells were cultured with 96-well plate under the following conditions.
CH150 medium is a medium that can lead to single cell growth for CHO-S cells and CHO-K1 cells.
＜ Culture conditions ＞
(1) Seeding cell density：1 cell/well.
(2) Static culture：37℃, 5% CO2.
(3) Culture volume：200 μL with L-glutamine.
The growth assay was performed using CH400AZ medium on clonal CHO-K1 cells obtained by cloning assay with CH150 medium.
Clonal cells had the ability to grow to higher cell densities compared to the cells before cloning assay.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Culture volume：30 mL with L-alanyl-L-glutamine.
The growth assay was performed using CH400AZ medium on clonal CHO-S cells obtained by cloning assay with CH150 medium.
Clonal cells had the ability to grow to higher cell densities compared to the cells before cloning assay.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Culture volume：30 mL with L-alanyl-L-glutamine.
Product Lineup
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