Gxpress 293 Enhancer has been developed for the gene expression system of human embryonic kidney (HEK) 293 cells, such as Expi293F or HEK293 cells, and the high production of recombinant proteins or the transfection assay in serum-free culture. Gxpress 293 Enhancer is a chemically defined composition that no contains serum, protein, animal origin, hydrolysates, and no unknown composition. In the transient transfection assay of 293 cells, Gxpress 293 Enhancer can increase the production of target recombinant protein by adding it to the medium.
Figure 1) Short-term prodiction of human IgG in Expi293F cells using the Gxpress 293 Transfection System.
Suspension Expi293F cells were cultured with disposable Erlenmeyer flasks under the following conditions. Gxpress 293 Transfection System leads to the high performance in human IgG (whole molecule) production for Expi293F cells. ＜ Culture conditions ＞ (1) Seeding cell density：2.94 x 10e6 cells/mL, >95% viability. (2) Shaker culture：125 rpm, 37℃, 8% CO2. (3) Transfection system：Gxpress 293 Transfection & Medium Kit (GMEP Inc.).
Figure 2) Short-term expression of β-galactosidase in Expi293F cells using the Gxpress 293 Transfection System.
Suspension Expi293F cells were cultured with disposable Erlenmeyer flasks under the following conditions. Gxpress 293 Transfection System leads to the high performance in β-galactosidase expression for Expi293F cells. ＜ Culture conditions ＞ (1) Seeding cell density：2.94 x 10e6 cells/mL, >95% viability. (2) Shaker culture：125 rpm, 37℃, 5% CO2. (3) Transfection system：Gxpress 293 Transfection & Medium Kit (GMEP Inc.).
Figure 3) Growth assay of 293 cells in HE400/HE400AZ medium.
Suspension 293 cells were cultured with disposable Erlenmeyer flasks under the following conditions. HE400/HE400AZ medium leads to the high performance similar to Thermo Expi293 Expression medium in cell growth for Expi293F cells. ＜ Culture conditions ＞ (1) Seeding cell density：3 x 10e5 cells/mL. (2) Shaker culture：125 rpm, 37℃, 8% CO2. (3) Culture volume：30 mL with L-alanyl-L-glutamine.
Figure 4) HE400/HE400AZ medium shows supperior performance in the cell culture of Expi293F cells.
Cryopreserved Expi293F cells were thawed using Thermo Expi293 Expression medium or HE400/HE400AZ medium, and subcultured in disposable Erlenmeyer flasks under the fillowing conditions. Next, the subculture was repeated with each medium to allow the cells to recover cryopreservation. Expi293F cells subcultured were subjected to growth assay using both Expi293 Expression medium and HE400/HE400AZ medium. Similar to the cells passaged in Expi293 Expression medium, Expi293F cells subcultured with HE400/HE400AZ medium can exert the high performance in cell culture. ＜ Culture conditions ＞ (1) Seeding cell density：3 x 10e5 cells/mL. (2) Shaker culture：125 rpm, 37℃, 8% CO2. (3) Culture volume：30 mL with L-alanyl-L-glutamine.
