Gxpress 293 Transfection & Medium Kit｜293 cells｜Transfection System

293 Transfection System

　Transfection Kit

【 Transfection Reagent & Medium Kit 】（Transfection Reagent + Enhancer Reagent + Chemically Defined Medium）

*It is a ready-to-use product including HE400AZ medium (with Ala-Gln) to which L-alanyl-L-glutamine is added as a product component.

❷ Gxpress 293 Transfection & Medium Kit II

*It is a product including HE400 medium (w/o Ala-Gln or Gln) that can control glutamine concentration during culture.

　Each Component

【 Transfection Reagent Kit 】（Transfection Reagent + Enhancer Reagent）

・Gxpress 293 Transfection KIt

【 Transfection Reagent 】

・Gxpress 293 TF Reagent

【 Enhancer Reagent 】

・Gxpress 293 Enhancer

【 Chemically Defined Medium 】

・HE400 medium

・HE400AZ medium

Transfection Reagent & Medium Kit
（Transfection Reagent + Enhancer Reagent + Chemically Defined Medium）
Gxpress 293 Transfection & Medium Kit
For 293 cells "Expi293F, 293-F, HEK293, 293T, VPC, etc."

293 transfection chemically defined serum free medium

　Gxpress 293 Transfection & Medium Kit has been developed for the gene expression system of human embryonic kidney (HEK) 293 cells, such as Expi293F or HEK293 cells, and the high production of recombinant proteins or the transfection assay in serum-free culture. All contents of Gxpress 293 Transfection & Medium Kit are a chemically defined composition that no contains serum, protein, animal origin, hydrolysates, and no unknown composition.
Gxpress 293 Transfection & Medium Kit consists of a highly efficient gene transfer reagent, 293 Complexing Solution to form optimal DNA/reagent complex, enhancer reagent to enhance recombinant protein production, and CD medium to lead to both high density culture and high production. With 293 cells and plasmid DNA, transfection assay for high production of target recombinant protein in a short term is possible.

Package Contents（Catalog Number GX293-MAK-0010）

・2.8 mL (1.4 mL x 2) Gxpress 293 TF Reagent

・100 mL (50 mL x 1) 293 Complexing Solution

・60 mL Gxpress 293 Enhancer

・1,000 mL HE400AZ medium (with Ala-Gln)

Protocol

・Quick Guide

・Gxpress 293 Kit Protocol

Figure 1) Short-term prodiction of human IgG in Expi293F cells using the Gxpress 293 Transfection System.

Suspension Expi293F cells were cultured with disposable Erlenmeyer flasks under the following conditions.
Gxpress 293 Transfection System leads to the high performance in human IgG (whole molecule) production for Expi293F cells.
＜ Culture conditions ＞
(1) Seeding cell density：2.94 x 10e6 cells/mL, >95% viability.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Transfection system：Gxpress 293 Transfection & Medium Kit (GMEP Inc.).

Figure 2) Short-term expression of β-galactosidase in Expi293F cells using the Gxpress 293 Transfection System.

Suspension Expi293F cells were cultured with disposable Erlenmeyer flasks under the following conditions.
Gxpress 293 Transfection System leads to the high performance in β-galactosidase expression for Expi293F cells.
＜ Culture conditions ＞
(1) Seeding cell density：2.94 x 10e6 cells/mL, >95% viability.
(2) Shaker culture：125 rpm, 37℃, 5% CO2.
(3) Transfection system：Gxpress 293 Transfection & Medium Kit (GMEP Inc.).

Figure 3) Growth assay of 293 cells in HE400/HE400AZ medium.

Suspension 293 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
HE400/HE400AZ medium leads to the high performance similar to Thermo Expi293 Expression medium in cell growth for Expi293F cells.
＜ Culture conditions ＞
(1) Seeding cell density：3 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Culture volume：30 mL with L-alanyl-L-glutamine.

Figure 4) HE400/HE400AZ medium shows supperior performance in the cell culture of Expi293F cells.

Cryopreserved Expi293F cells were thawed using Thermo Expi293 Expression medium or HE400/HE400AZ medium, and subcultured in disposable Erlenmeyer flasks under the fillowing conditions.
Next, the subculture was repeated with each medium to allow the cells to recover cryopreservation. Expi293F cells subcultured were subjected to growth assay using both Expi293 Expression medium and HE400/HE400AZ medium.
Similar to the cells passaged in Expi293 Expression medium, Expi293F cells subcultured with HE400/HE400AZ medium can exert the high performance in cell culture.
＜ Culture conditions ＞
(1) Seeding cell density：3 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Culture volume：30 mL with L-alanyl-L-glutamine.