293 Medium Lineup
293 Chemically Defined Media
HE100 medium
① Adherent Culture(293 cells;Expi293F, 293-F, HEK293, 293T, etc.)
・For 293 cells such as Expi293F, 293-F, HEK293, 293T cells, etc.
・Chemically defined medium / Serum-free medium.
・Medium optimized for transfection system.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.
HE150 medium
② Cloning Assay(293 cells;Expi293F, 293-F, HEK293, 293T, etc.)
・For 293 cells such as Expi293F, 293-F, HEK293, 293T cells, etc.
・Chemically defined medium / Serum-free medium.
・Medium optimized for a single cell cloninng for 293 cells.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.
HE400AZ medium
③ Suspension Culture(293 cells;Expi293F, 293-F, HEK293, 293T, etc.)
・For 293 cells such as Expi293F, 293-F, HEK293, 293T cells, etc.
・High level growth & nutrient rich medium for 293 cells.
・Medium optimized for protein production with high density culture.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.
HE400/AZ medium, HE300/AZ medium, HE200 medium
Suspension Culture(293 cells;Expi293F, 293-F, HEK293, 293T, etc.)
・The line up of three grades. You can select the optimal medium according to the test scenes using 293 cells.
Chemically Defined Medium (293 cells) |
HE200 medium |
HE300/AZ medium | HE400/AZ medium | ||
HE300 | HE300AZ | HE400 | HE400AZ | ||
L-Glutamine | − | − | − | − | − |
L-Alanyl-L-glutamine | − | − | ● | − | ● |
Nutrition ratio(composition) | 1.0 | 1.1 | 1.6 | ||
Final cell density(cells/mL)*1 | 3.0−6.0 x 10e6 | 4.0−7.0 x 10e6 | 7.0−10 x 10e6 | ||
Transfection assay *2 | ● | ● | ● | ||
Adaptation culture *3 | ● | ● | ● | ||
Subculture(>100 days) | ● | ● | ● | ||
Fed-batch culture *4 | ● | ● | ● | ||
Price(1,000 mL) | ¥9,800 | ¥12,900 | ¥13,900 | ¥19,600 | ¥21,600 |
*1 Growth assay of Expi293F, 293T, 293-F, and HEK293 cells. *2 Transfection assay with Gxpress 293 Transfection kit using Expi293F, 293T, 293-F, and HEK293 cells. *3 Adaptation culture of Expi293F, 293T, 293-F, and HEK293 cells. *4 Fed-batch culture with Gxpress 293 Feed medium using Expi293F, 293T, 293-F, and HEK293 cells. |
Gxpress 293 Feed medium
④ Fed-Batch Culture(293 cells;Expi293F, 293-F, HEK293, 293T, etc.)
・For 293 cells such as Expi293F, 293-F, HEK293, 293T cells, etc.
・Medium optimized for protein production with high density culture.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.
Product Lineup

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Hyakunen Park, Kurume, Fukuoka, Japan