293 Medium Lineup
293 Chemically Defined Media
HE100 medium
① Adherent Culture（293 cells；Expi293F, 293-F, HEK293, 293T, VPC, etc.）
・For 293 cells such as Expi293F, 293-F, HEK293, 293T, VPC cells, etc.
・Chemically defined medium / Serum-free medium.
・Medium optimized for transfection system.
・Medium contains no proteins, hydrolysates, or human/animal-derived components.
HE150 medium
② Cloning Assay（293 cells；Expi293F, 293-F, HEK293, 293T, VPC, etc.）
・For 293 cells such as Expi293F, 293-F, HEK293, 293T, VPC cells, etc.
・Chemically defined medium / Serum-free medium.
・Medium optimized for a single cell cloninng for 293 cells.
・Medium contains no proteins, hydrolysates, or human/animal-derived components.
HE400AZ medium
③ Suspension Culture（293 cells；Expi293F, 293-F, HEK293, 293T, VPC, etc.）
・For 293 cells such as Expi293F, 293-F, HEK293, 293T, VPC cells, etc.
・High level growth & nutrient rich medium for 293 cells.
・Medium optimized for protein production with high density culture.
・Medium contains no proteins, hydrolysates, or human/animal-derived components.
HE400/AZ medium, HE300/AZ medium, HE200 medium
Suspension Culture（293 cells；Expi293F, 293-F, HEK293, 293T, VPC, etc.）
・The line up of three grades. You can select the optimal medium according to the test scenes using 293 cells.
| Chemically Defined Medium （293 cells） |
HE200 medium |
HE300/AZ medium | HE400/AZ medium | ||
| HE300 | HE300AZ | HE400 | HE400AZ | ||
| L-Glutamine | − | − | − | − | − |
| L-Alanyl-L-glutamine | − | − | ● | − | ● |
| Nutrition ratio（composition） | 1.0 | 1.1 | 1.6 | ||
| Final cell density（cells/mL）*1 | 3.0−6.0 x 10e6 | 4.0−7.0 x 10e6 | 7.0−10 x 10e6 | ||
| Transfection assay *2 | ● | ● | ● | ||
| Adaptation culture *3 | ● | ● | ● | ||
| Subculture（>100 days） | ● | ● | ● | ||
| Fed-batch culture *4 | ● | ● | ● | ||
| Price（1,000 mL） | ¥9,800 | ¥12,900 | ¥13,900 | ¥19,600 | ¥21,600 |
| *1 Growth assay of Expi293F, 293T, 293-F, HEK293, and VPC cells. *2 Transfection assay with Gxpress 293 Transfection kit using Expi293F, 293T, 293-F, HEK293, and VPC cells. *3 Adaptation culture of Expi293F, 293T, 293-F, HEK293, and VPC cells. *4 Fed-batch culture with Gxpress 293 Feed medium using Expi293F, 293T, 293-F, HEK293, and VPC cells. |
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Gxpress 293 Feed medium
④ Fed-Batch Culture（293 cells；Expi293F, 293-F, HEK293, 293T, VPC, etc.）
・For 293 cells such as Expi293F, 293-F, HEK293, 293T, VPC cells, etc.
・Medium optimized for protein production with high density culture.
・Medium contains no proteins, hydrolysates, or human/animal-derived components.
Product Lineup
Gmep Incorporated
Hyakunen Park, Kurume, Fukuoka, Japan