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293 Medium Lineup

293 Chemically Defined Media

① Adherent Culture
 
② Cloning Assay
③ Suspension Culture
*Ready-to-use products with L-alanyl-L-glutamine
④ Fed-Batch Culture

HE100 medium

① Adherent Culture(293 cells;Expi293F, 293-F, 293T, HEK293, etc.)


・For 293 cells such as Expi293F, 293-F, 293T, HEK293 cells, etc.
・Chemically defined medium / Serum-free medium.
・Medium optimized for transfection system.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.


293 cells|Single cell cloning assay|Chemically defined & Serum-free medium

HE150 medium

② Cloning Assay(293 cells;Expi293F, 293-F, 293T, HEK293, etc.)


・For 293 cells such as Expi293F, 293-F, 293T, HEK293 cells, etc.
・Chemically defined medium / Serum-free medium.
・Medium optimized for a single cell cloninng for 293 cells.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.


293 cells|Suspension culture|Chemically defined & Serum-free medium

HE400AZ medium

③ Suspension Culture(293 cells;Expi293F, 293-F, 293T, HEK293, etc.)


・For 293 cells such as Expi293F, 293-F, 293T, HEK293 cells, etc.
・High level growth & nutrient rich medium for 293 cells.
・Medium optimized for protein production with high density culture.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.

HE400/AZ medium, HE300/AZ medium, HE200 medium

Suspension Culture(293 cells;Expi293F, 293-F, 293T, HEK293, etc.)
・The line up of three grades. You can select the optimal medium according to the test scenes using 293 cells.

 Chemically Defined Medium
 (293 cells)
HE200
medium
HE300/AZ medium HE400/AZ medium
HE300 HE300AZ HE400 HE400AZ
  L-Glutamine
  L-Alanyl-L-glutamine
  Nutrition ratio(composition) 1.0 1.1 1.6
  Final cell density(cells/mL)*1 3.0−6.0 x 10e6 4.0−7.0 x 10e6 7.0−10 x 10e6
  Transfection assay  *2
  Adaptation culture  *3
  Subculture(>100 days)
  Fed-batch culture  *4
  Price(1,000 mL) ¥9,800 ¥12,900 ¥13,900 ¥19,600 ¥21,600
*1   Growth assay of Expi293F, 293T, 293-F, and HEK293 cells.
*2   Transfection assay with Gxpress 293 Transfection kit using Expi293F, 293T, 293-F, and HEK293 cells.
*3   Adaptation culture of Expi293F, 293T, 293-F, and HEK293 cells.
*4   Fed-batch culture with Gxpress 293 Feed medium using Expi293F, 293T, 293-F, and HEK293 cells.


293 cells|Feed medium|Chemically defined & Serum-free medium

Gxpress 293 Feed medium

④ Fed-Batch Culture(293 cells;Expi293F, 293-F, 293T, HEK293, etc.)


・For 293 cells such as Expi293F, 293-F, 293T, HEK293 cells, etc.
・Medium optimized for protein production with high density culture.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.


Product Lineup

Gmep Incorporated

Hyakunen Park, Kurume, Fukuoka, Japan