293 Medium Lineup
293 Chemically Defined Media
HE100 medium
① Adherent Culture（293 cells；Expi293F, 293-F, HEK293, 293T, etc.）
・For 293 cells such as Expi293F, 293-F, HEK293, 293T cells, etc.
・Chemically defined medium / Serum-free medium.
・Medium optimized for transfection system.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.
HE150 medium
② Cloning Assay（293 cells；Expi293F, 293-F, HEK293, 293T, etc.）
・For 293 cells such as Expi293F, 293-F, HEK293, 293T cells, etc.
・Chemically defined medium / Serum-free medium.
・Medium optimized for a single cell cloninng for 293 cells.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.
HE400AZ medium
③ Suspension Culture（293 cells；Expi293F, 293-F, HEK293, 293T, etc.）
・For 293 cells such as Expi293F, 293-F, HEK293, 293T cells, etc.
・High level growth & nutrient rich medium for 293 cells.
・Medium optimized for protein production with high density culture.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.
HE400/AZ medium, HE300/AZ medium, HE200 medium
Suspension Culture（293 cells；Expi293F, 293-F, HEK293, 293T, etc.）
・The line up of three grades. You can select the optimal medium according to the test scenes using 293 cells.
| Chemically Defined Medium （293 cells） |
HE200 medium |
HE300/AZ medium | HE400/AZ medium | ||
| HE300 | HE300AZ | HE400 | HE400AZ | ||
| L-Glutamine | − | − | − | − | − |
| L-Alanyl-L-glutamine | − | − | ● | − | ● |
| Nutrition ratio（composition） | 1.0 | 1.1 | 1.6 | ||
| Final cell density（cells/mL）*1 | 3.0−6.0 x 10e6 | 4.0−7.0 x 10e6 | 7.0−10 x 10e6 | ||
| Transfection assay *2 | ● | ● | ● | ||
| Adaptation culture *3 | ● | ● | ● | ||
| Subculture（>100 days） | ● | ● | ● | ||
| Fed-batch culture *4 | ● | ● | ● | ||
| Price（1,000 mL） | ¥9,800 | ¥12,900 | ¥13,900 | ¥19,600 | ¥21,600 |
| *1 Growth assay of Expi293F, 293T, 293-F, and HEK293 cells. *2 Transfection assay with Gxpress 293 Transfection kit using Expi293F, 293T, 293-F, and HEK293 cells. *3 Adaptation culture of Expi293F, 293T, 293-F, and HEK293 cells. *4 Fed-batch culture with Gxpress 293 Feed medium using Expi293F, 293T, 293-F, and HEK293 cells. |
|||||
Gxpress 293 Feed medium
④ Fed-Batch Culture（293 cells；Expi293F, 293-F, HEK293, 293T, etc.）
・For 293 cells such as Expi293F, 293-F, HEK293, 293T cells, etc.
・Medium optimized for protein production with high density culture.
・Medium contains no proteins, hydrolysates, or components of animal and plant composition.
Product Lineup
Gmep Incorporated
Hyakunen Park, Kurume, Fukuoka, Japan