Enhancer Reagent Gxpress CHO Enhancer For CHO cells "CHO-K1, CHO-S, DG44, DXB11, etc."
Gxpress CHO Enhancer has been developed for the gene expression system of Chinese hamster overy (CHO) cells, such as CHO-S, CHO-K1, DG44, or DXB11 cells, and the high production of recombinant proteins or the transfection assay in serum-free culture. Gxpress CHO Enhancer is a chemically defined composition that no contains serum, protein, animal origin, hydrolysates, and no unknown composition. In the transient transfection assay of CHO cells, Gxpress CHO Enhancer can increase the production of target recombinant protein by adding it to the medium.
Figure 1) Short-term expression of human IgG in CHO cells using the Gxpress CHO Transfection System.
Suspension CHO cells were cultured with disposable Erlenmeyer flasks under the following conditions. Gxpress CHO Transfection System leads to the high performance in human IgG (whole molecule) production for CHO cells. ＜ Culture conditions ＞ (1) Seeding cell density：2 x 10e6 cells/mL, >95% viability. (2) Shaker culture：125 rpm, 37℃, 8% CO2. (3) Transfection system：Gxpress CHO Transfection & Medium Kit (GMEP Inc.).
Figure 2) Growth assay of CHO cells with CH400/CH400AZ medium.
Suspension CHO cells were cultured with disposable Erlenmeyer flasks under the following conditions. CH400/400AZ medium leads to the high performance similar to Thermo CD OptiCHO medium in cell growth for CHO cells. ＜ Culture conditions ＞ (1) Seeding cell density：2 x 10e5 cells/mL. (2) Shaker culture：125 rpm, 37℃, 8% CO2. (3) Culture volume：30 mL with L-alanyl-L-glutamine.
