Gxpress CHO Transfection & Medium Kit II｜CHO cells｜Transfection System

CHO Transfection System

　Transfection Kit

【 Transfection Reagent & Medium Kit 】（Transfection Reagent + Enhancer Reagent + Chemically Defined Medium）

*It is a ready-to-use product including CH400AZ medium (with Ala-Gln) to which L-alanyl-L-glutamine is added as a product component.

❷ Gxpress CHO Transfection & Medium Kit II

*It is a product including CH400 medium (w/o Ala-Gln or Gln) that can control glutamine concentration during culture.

　Each Component

【 Transfection Reagent Kit 】（Transfection Reagent + Enhancer Reagent）

・Gxpress CHO Transfection KIt

【 Transfection Reagent 】

・Gxpress CHO TF Reagent

【 Enhancer Reagent 】

・Gxpress CHO Enhancer

【 Chemically Defined Medium 】

・CH400 medium

・CH400AZ medium

Transfection Reagent & Medium Kit
（Transfection Reagent + Enhancer Reagent + Chemically Defined Medium）
Gxpress CHO Transfection & Medium Kit II
For CHO cells "CHO-K1, CHO-K1, DG44, DXB11, etc."

CHO transfection chemically defined serum free medium

　Gxpress CHO Transfection & Medium Kit II has been developed for the gene expression system of Chinese hamster overy (CHO) cells, such as CHO-K1 or CHO-S cells, and the high production of recombinant proteins or the transfection assay in serum-free culture. All contents of Gxpress CHO Transfection & Medium Kit are a chemically defined composition that no contains serum, protein, animal origin, hydrolysates, and no unknown composition.
Gxpress CHO Transfection & Medium Kit II consists of a highly efficient gene transfer reagent, CHO Complexing Solution to form optimal DNA/reagent complex, enhancer reagent to enhance recombinant protein production, and CD medium to lead to both high density culture and high production. With CHO cells and plasmid DNA, transfection assay for high production of target recombinant protein in a short term is possible.

Package Contents (Catalog Number GXCHO-MK-0010)

・2.4 mL (1.2 mL x 2) Gxpress CHO TF Reagent

・80 mL (40 mL x 2) CHO Complexing Solution

・120 mL Gxpress CHO Enhancer

・1,000 mL CH400 medium (w/o Ala-Gln or Gln)

Protocol

・Quick Guide

・Gxpress CHO Kit Protocol

Figure 1) Short-term expression of human IgG in CHO cells using the Gxpress CHO Transfection System.

Suspension CHO cells were cultured with disposable Erlenmeyer flasks under the following conditions.
Gxpress CHO Transfection System leads to the high performance in human IgG (whole molecule) production for CHO cells.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e6 cells/mL, >95% viability.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Transfection system：Gxpress CHO Transfection & Medium Kit (GMEP Inc.).

Figure 2) Growth assay of CHO cells with CH400/CH400AZ medium.

Suspension CHO cells were cultured with disposable Erlenmeyer flasks under the following conditions.
CH400/400AZ medium leads to the high performance similar to Thermo CD OptiCHO medium in cell growth for CHO cells.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Culture volume：30 mL with L-alanyl-L-glutamine.