HOME | Gxpress 293 Feed medium|293 cells|Fed-Batch Culture|Chemically Defined Medium

293 Chemically Defined Media

① Adherent Culture
 
② Cloning Assay
③ Suspension Culture
*Ready-to-use products with L-alanyl-L-glutamine
④ Fed-Batch Culture

Fed-Batch Culture (Expression & Production)
Gxpress 293 Feed medium (CD medium), liquid or powder
For 293 cells "Expi293F, 293-F, HEK293, 293T, VPC, etc."

293 cells|Feed medium|Chemically defined & Serum-free medium

 Gxpress 293 Feed medium is a chemically defined medium / serum-free medium for using in fed-batch culture and optimized for the high production of recombinant proteins in human embryonic kidney (HEK) 293 cells, such as Expi293F, 293-F, HEK293, 293T, or VPC cells. The medium contains no proteins, hydrolysates, or human/animal-derived components. All components have a known chemical structure.

   Gxpress 293 Feed medium has the advantages of lot-to-lot consistency and the ease of downstream purification after expression of recombinant proteins. The feed medium supports small- and large-scale protein expression in stably or transiently transfected 293 cells.

Fig. 1) Growth rate of Expi293F cells in HE300AZ medium with Gxpress 293 Feed medium.

   Expi293F cells adapted to HE300AZ medium were incubated at a cell density of 3.0 x 10e5 cells/mL with HE300AZ medium (30 mL) in 125 mL sharker flasks and rotated at 125 rpm, 37℃, and 5% CO2. Gxpress 293 Feed medium was added on day 3 at 3 mL.

Fig. 2) Growth rate of 293T cells in HE300AZ medium with Gxpress 293 Feed medium.

   293T cells adapted to HE300AZ medium were incubated at a cell density of 3.0 x 10e5 cells/mL with HE300AZ medium (30 mL) in 125 mL sharker flasks and rotated at 125 rpm, 37℃, and 5% CO2. Gxpress 293 Feed medium was added on day 3 at 3 mL.

Fig. 3) Growth rate of Expi293F cells in HE200 medium with Gxpress 293 Feed medium.

   Expi293F cells adapted to HE300AZ medium were incubated at a cell density of 3.0 x 10e5 cells/mL with HE200 medium (30 mL with 2 mM Ala-Gln) in 125 mL sharker flasks and rotated at 125 rpm, 37℃, and 5% CO2. Gxpress 293 Feed medium was added on day 3 at 3 mL.


Product Lineup

Gmep Incorporated

Hyakunen Park, Kurume, Fukuoka, Japan