293 Chemically Defined Media
Suspension Culture (Expression & Production)
HE300 medium (CD medium), liquid or powder
For 293 cells "Expi293F, 293-F, HEK293, 293T, VPC, etc."
HE300 medium is a chemically defined medium / serum-free medium optimized for the high production of recombinant proteins in human embryonic kidney (HEK) 293 cells, such as Expi293F, 293-F, HEK293, 293T, or VPC cells. The medium contains no proteins, hydrolysates, or human/animal-derived components. All components have a known chemical structure.
Figure 1) Growth assay of Expi293F cells in HE300/HE300AZ medium.
Suspension 293 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
HE300/HE300AZ medium leads to the high performance similar to Thermo FreeStyle 293 medium in cell growth for Expi293F cells.
< Culture conditions >
(1) Seeding cell density:3 x 10e5 cells/mL.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Culture volume:30 mL with L-alanyl-L-glutamine.
Figure 2) Growth assay of 293-F cells in HE300/HE300AZ medium.
Suspension 293 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
HE300/HE300AZ medium leads to the high performance similar to Thermo FreeStyle 293 medium in cell growth for 293-F cells.
< Culture conditions >
(1) Seeding cell density:3 x 10e5 cells/mL.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Culture volume:30 mL with L-alanyl-L-glutamine.
HE300/HE300AZ medium exhibits the equal or higher performance in human IgG production compared to Thermo Expi293 Expression medium without inhibiting the transfection assay.
< Culture conditions >
(1) Seeding cell density:2.94 x 10e6 cells/mL, >95% viability.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Transfection reagent:Gxpress 293 TF Reagent (GMEP Inc.).
HE300/HE300AZ medium exhibits the equal or higher performance in gene expression compared to Thermo Expi293 Expression medium without inhibiting the transfection assay.
< Culture conditions >
(1) Seeding cell density:2.94 x 10e6 cells/mL, >95% viability.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Transfection reagent:Gxpress 293 TF Reagent (GMEP Inc.).
Figure 5) Growth assay of 293T cells or HEK293 cells in HE300/HE300AZ medium.
Suspension 293 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
HE300/HE300AZ medium leads to the high performance similar to Thermo FreeStyle 293 medium in cell growth for 293T cells or HEK293 cells.
These cells cultured in DMEM with 10% FBS are suspended with adaptation in HE400AZ medium.
< Culture conditions >
(1) Seeding cell density:3 x 10e5 cells/mL.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Culture volume:30 mL with L-alanyl-L-glutamine.
Product Lineup
Gmep Incorporated
Hyakunen Park, Kurume, Fukuoka, Japan