HOME | HE300 medium|293 cells|Suspension Culture|Chemically Defined Medium

293 Chemically Defined Media

① Adherent Culture
 
② Cloning Assay
③ Suspension Culture
*Ready-to-use products with L-alanyl-L-glutamine
④ Fed-Batch Culture

Suspension Culture (Expression & Production)
HE300 medium (CD medium), liquid or powder
For 293 cells "Expi293F, 293-F, HEK293, 293T, VPC, etc."

293 cells|Suspension culture|Chemically defined & Serum-free medium

 HE300 medium is a chemically defined medium / serum-free medium optimized for the high production of recombinant proteins in human embryonic kidney (HEK) 293 cells, such as Expi293F, 293-F, HEK293, 293T, or VPC cells. The medium contains no proteins, hydrolysates, or human/animal-derived components. All components have a known chemical structure.

   HE300 medium has the advantages of lot-to-lot consistency and the ease of downstream purification after expression of recombinant proteins. The medium supports small- and large-scale protein expression in stably or transiently transfected 293 cells.
Protocol

Figure 1) Growth assay of Expi293F cells in HE300/HE300AZ medium.


Suspension 293 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
HE300/HE300AZ medium leads to the high performance similar to Thermo FreeStyle 293 medium in cell growth for Expi293F cells.
< Culture conditions >
(1) Seeding cell density:3 x 10e5 cells/mL.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Culture volume:30 mL with L-alanyl-L-glutamine.


Figure 2) Growth assay of 293-F cells in HE300/HE300AZ medium.


Suspension 293 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
HE300/HE300AZ medium leads to the high performance similar to Thermo FreeStyle 293 medium in cell growth for 293-F cells.
< Culture conditions >
(1) Seeding cell density:3 x 10e5 cells/mL.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Culture volume:30 mL with L-alanyl-L-glutamine.


Figure 3) Transient transfection assay of Expi293F cells with HE300/HE300AZ medium.

HE300/HE300AZ medium exhibits the equal or higher performance in human IgG production compared to Thermo Expi293 Expression medium without inhibiting the transfection assay.
< Culture conditions >
(1) Seeding cell density:2.94 x 10e6 cells/mL, >95% viability.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Transfection reagent:Gxpress 293 TF Reagent (GMEP Inc.).


Figure 4) Transient transfection assay of Expi293F cells with HE300/HE300AZ medium.

HE300/HE300AZ medium exhibits the equal or higher performance in gene expression compared to Thermo Expi293 Expression medium without inhibiting the transfection assay.
< Culture conditions >
(1) Seeding cell density:2.94 x 10e6 cells/mL, >95% viability.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Transfection reagent:Gxpress 293 TF Reagent (GMEP Inc.).


Figure 5) Growth assay of 293T cells or HEK293 cells in HE300/HE300AZ medium.


Suspension 293 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
HE300/HE300AZ medium leads to the high performance similar to Thermo FreeStyle 293 medium in cell growth for 293T cells or HEK293 cells.
These cells cultured in DMEM with 10% FBS are suspended with adaptation in HE400AZ medium.
< Culture conditions >
(1) Seeding cell density:3 x 10e5 cells/mL.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Culture volume:30 mL with L-alanyl-L-glutamine.


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