293 Chemically Defined Media
Adherent Culture (Expression & Production)
HE100 medium (CD medium), liquid or powder
For 293 cells "Expi293F, 293-F, HEK293, 293T, etc."
HE100 medium is a chemically defined medium / serum-free medium optimized for the adherent growth of human embryonic kidney (HEK) 293 cells, such as Expi293F, 293-F, HEK293, or 293T cells. The medium contains no proteins, hydrolysates, or components of animal and plant composition. All components have a known chemical structure.
HE100 medium has the advantages of lot-to-lot consistency and the ease of downstream purification after expression of recombinant proteins. The medium supports small- and large-scale protein expression in stably or transiently transfected 293 cells.
Human 293 cells were cultured with 12-well plate under the following conditions.
These cells were adaptated with GMEP CD medium.
＜ Culture conditions ＞
(1) Seeding cell density：1 x 10e5 cells/mL.
(2) Static culture：37℃, 8% CO2.
(3) Culture volume：2 mL with L-alanyl-L-glutamine.
Hyakunen Park, Kurume, Fukuoka, Japan