HE100 medium｜293 cells｜Adherent Culture｜Chemically Defined Medium

293 Chemically Defined Media

① Adherent Culture

・HE100 medium

② Cloning Assay

・HE150 medium

③ Suspension Culture

・HE200 medium

・HE300 medium

・HE300AZ medium＊

・HE400 medium

・HE400AZ medium＊

＊Ready-to-use products with L-alanyl-L-glutamine

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④ Fed-Batch Culture

・Gxpress 293 Feed medium

Adherent Culture (Expression & Production)
HE100 medium (CD medium), liquid or powder
For 293 cells "Expi293F, 293-F, HEK293, 293T, VPC, etc."

293 chemically defined serum free medium

HE100 medium is a chemically defined medium / serum-free medium optimized for the adherent growth of human embryonic kidney (HEK) 293 cells, such as Expi293F, 293-F, HEK293, 293T, or VPC cells. The medium contains no proteins, hydrolysates, or human/animal-derived components. All components have a known chemical structure.
HE100 medium has the advantages of lot-to-lot consistency and the ease of downstream purification after expression of recombinant proteins. The medium supports small- and large-scale protein expression in stably or transiently transfected 293 cells.

Protocol

・HE100 medium protocol

Figure) Growth assay of 293 cells with HE100 medium on adherent culture.

Human 293 cells were cultured with 12-well plate under the following conditions.
These cells were adaptated with GMEP CD medium.
＜ Culture conditions ＞
(1) Seeding cell density：1 x 10e5 cells/mL.
(2) Static culture：37℃, 8% CO2.
(3) Culture volume：2 mL with L-alanyl-L-glutamine.