Chemically Defined Media (Fibroblast Hybridoma Myeloma)
Hybridoma & Myeloma (Monoclonal Antibody Production)
HY300 medium (CD medium), liquid or powder
For hybridomas and myelomas
HY300 medium is a chemically defined medium / serum-free medium optimized for the growth and antibody production of hybridoma or myeloma in serum-free culture. The medium contains phenol red, but no proteins, hydrolysates, or human/animal-derived components. All components have a known chemical structure.
HY300 medium has the advantages of lot-to-lot consistency and the ease of downstream purification of target proteins. When culturing cholesterol-dependent myelomas such as NS0 cells, it is necessary to add cell culture tested cholesterol into the medium.
Fig. 1）Growth rate of mouse hybridomas in HY300 medium or commercial media.
Mouse hybridoma 9E10 cells subcultured in IMDM with 10% FBS were directly inoculated at 2.0 x 10e5 cells/mL and incubated into HY300 medium (5 mL with 4 mM Gln), IMDM with 10% FBS (5 mL), or other commercial media (5 mL with 4 mM Gln) in T25 flasks at 37℃ and 5% CO2.
Fig. 2）Growth rate of rat hybridomas in HY300 medium or commercial media.
Rat hybridoma BF11 cells subcultured in IMDM with 10% FBS were directly inoculated at 2.0 x 10e5 cells/mL and incubated into HY300 medium (5 mL with 4 mM Gln), IMDM with 10% FBS (5 mL), or other commercial media (5 mL with 4 mM Gln) in T25 flasks at 37℃ and 5% CO2.
Fig. 3）Growth rate of mouse hybridomas adaptated in serum-free medium.
Mouse hybridoma 9E10 cells subcultured in HY300 medium were inoculated at 2.0 x 10e5 cells/mL and incubated into HY300 medium (5 mL with 4 mM Gln) or other commercial media (5 mL with 4 mM Gln) in T25 flasks at 37℃ and 5% CO2.
Fig. 4）Growth rate of rat hybridomas adaptated in HY300 medium.
Rat hybridoma BF11 cells subcultured in HY300 medium were inoculated at 2.0 x 10e5 cells/mL and incubated into HY300 medium (5 mL with 4 mM Gln) or other commercial media (5 mL with 4 mM Gln) in T25 flasks at 37℃ and 5% CO2.
Fig. 5）Growth rate of mouse myelomas in HY300 medium or commercial media.
Mouse myeloma NS0 cells were inoculated at 2.0 x 10e5 cells/mL and incubated into HY300 medium (5 mL with 4 mM Gln), IMDM with 10% FBS (5 mL), or other commercial media (5 mL with 4 mM Gln) in T25 flasks at 37℃ and 5% CO2.
Product Lineup
Gmep Incorporated
Hyakunen Park, Kurume, Fukuoka, Japan