Adherent Culture (Expression ＆ Production) CH100 medium (CD medium), liquid or powder For CHO cells "CHO-K1, CHO-S, DG44, DXB11, etc."
CH100 medium is a chemically defined medium / serum-free medium optimized for the adherent growth of Chinese hamster ovary (CHO) cells, such as CHO-K1, CHO-S, DG44, and DXB11 cells. The medium contains no proteins, hydrolysates, or human/animal-derived components. All components have a known chemical structure. CH100 medium has the advantages of lot-to-lot consistency and the ease of downstream purification after expression of recombinant proteins. The medium supports small- and large-scale protein expression in stably or transiently transfected CHO cells.
Figure) Growth assay of CHO cells with CH100 medium on adherent culture.
CHO cells were cultured with 12-well plate under the following conditions. These cells were adaptated with GMEP CD medium. ＜ Culture conditions ＞ (1) Seeding cell density：1 x 10e5 cells/mL. (2) Static culture：37℃, 8% CO2. (3) Culture volume：2 mL with L-alanyl-L-glutamine.
