CHO Chemically Defined Media
Anti-Clumping Reagent
Scattering reagent CHO (CD reagent)
For CHO cells "CHO-K1, CHO-S, DG44, DXB11, etc."
Scattering reagent CHO is a chemically defined reagent to suppress cell clumping in serum-free culture of Chinese hamster ovary (CHO) cells, such as CHO-K1, CHO-S, DG44, and DXB11 cells. The reagent contains no proteins, hydrolysates, or components of animal and plant composition. All components have a known chemical structure.
Cell aggregation leads to a decrease in recombinant protein production as well as a reduction in cell viability. Scattering reagent CHO can reduce cell clumping only by adding to the culture medium in shaker culture or static culture.
Fig. 1) Cell clumping suppression of CHO-S cells with Scattering reagent CHO.
CHO-S cells cultured with serum-free medium were inoculated at a cell density of 2.0 x 10e5 cells/mL and incubated for 2 days with serum-free medium (30 mL with 8 mM Gln and Scattering reagent CHO at 1:200 dilution) in 125 mL sharker flasks at 125 rpm, 37℃, and 5% CO2.
Fig. 2）Cell clumping generation of CHO-S cells on serum-free medium.
CHO-S cells cultured with serum-free medium were inoculated at a cell density of 2.0 x 10e5 cells/mL and incubated for 2 days with serum-free medium (30 mL with 8 mM Gln) in 125 mL sharker flasks at 125 rpm, 37℃, and 5% CO2.
Fig. 3) Cell clumping suppression of CHO-DXB11 cells with Scattering reagent CHO.
CHO-DXB11 cells cultured with serum-free medium were inoculated at a cell density of 2.0 x 10e5 cells/mL and incubated for 2 days with serum-free medium (30 mL with 8 mM Gln and Scattering reagent CHO at 1:200 dilution) in 125 mL sharker flasks at 125 rpm, 37℃, and 5% CO2.
Fig. 4）Cell clumping generation of CHO-DXB11 cells on serum-free medium.
CHO-DXB11 cells cultured with serum-free medium were inoculated at a cell density of 2.0 x 10e5 cells/mL and incubated for 2 days with serum-free medium (30 mL with 8 mM Gln) in 125 mL sharker flasks at 125 rpm, 37℃, and 5% CO2.
Fig. 5) Growth rate of CHO-S cells with the addition of Scattering reagent CHO.
CHO-S cells cultured with serum-free medium were inoculated at a cell density of 2.0 x 10e5 cells/mL and incubated with CH300 medium (30 mL with 8 mM Gln and Scattering reagent CHO at 1:200 dilution) in 125 mL sharker flasks at 125 rpm, 37℃, and 5% CO2.
Fig. 6）Growth rate of CHO-DG44 cells with the addition of Scattering reagent CHO.
CHO-DG44 cells cultured with serum-free medium were inoculated at a cell density of 2.0 x 10e5 cells/mL and incubated with CH300 medium (30 mL with 8 mM Gln and Scattering reagent CHO at 1:200 dilution) in 125 mL sharker flasks at 125 rpm, 37℃, and 5% CO2.
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