CH200 medium｜CHO cells｜Suspension Culture｜Chemically Defined Medium

CHO Chemically Defined Media

① Adherent Culture

・CH100 medium

② Cloning Assay

・CH150 medium

③ Suspension Culture

・CH200 medium

・CH300 medium

・CH300AZ medium＊

・CH400 medium

・CH400AZ medium＊

＊Ready-to-use products with L-alanyl-L-glutamine

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④ Fed-Batch Culture

・Gxpress CHO Feed medium

⑤ Anti-Clumping Reagent

・Scattering reagent CHO

Suspension Culture (Expression & Production)
CH200 medium (CD medium), liquid or powder
For CHO cells "CHO-K1, CHO-S, DG44, DXB11, etc."

CHO cells｜Suspension culture｜Chemically defined & Serum-free medium

　CH200 medium is a chemically defined medium / serum-free medium optimized for the high production of recombinant proteins in Chinese hamster ovary (CHO) cells, such as CHO-K1, CHO-S, DG44, and DXB11 cells. The medium contains no proteins, hydrolysates, or human/animal-derived components. All components have a known chemical structure.

CH200 medium has the advantages of lot-to-lot consistency and the ease of downstream purification after expression of recombinant proteins. The medium supports small- and large-scale protein expression in stably or transiently transfected CHO cells.

Protocol

・CH200 medium protocol

Figure 1) Growth assay of CHO-K1 cells with CH200 medium.

Suspension CHO-K1 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
CH200 medium leads to the high performance similar to Thermo CD OptiCHO medium in cell growth for CHO-K1 cells.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Culture volume：30 mL with L-alanyl-L-glutamine.

Figure 2) Growth assay of CHO-S cells with CH200 medium.

Suspension CHO-S cells were cultured with disposable Erlenmeyer flasks under the following conditions.
CH200 medium leads to the high performance similar to Thermo CD OptiCHO medium in cell growth for CHO-S cells.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Culture volume：30 mL with L-alanyl-L-glutamine.

Figure 3) Transient transfection assay of CHO-K1 cells with CH200 medium.

CH200 medium exhibits the higher performance in human IgG production compared to other company medium without inhibiting the transfection assay.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e6 cells/mL, >95% viability.
(2) Static culture：37℃, 5% CO2.

Figure 4) Transient transfection assay of CHO cells with CH200 medium.

CH200 medium exhibits the equal or higher performance in gene expression compared to other campany medium without inhibiting the transfection assay.
＜ Culture conditions ＞
(1) Seeding cell density：6 x 10e6 cells/mL, >95% viability.
(2) Shaker culture：125 rpm, 37℃, 5% CO2.
(3) Transfection reagent：Gxpress CHO TF Reagent (GMEP Inc.).

Figure 5）Growth assay of CHO-DG44 cells with CH200 medium.

Suspension CHO-DG44 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
CH200 medium leads to good cell growth for CHO-DG44 cells.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 5% CO2.
(3) Culture volume：30 mL with L-glutamine.