CH300 medium｜CHO cells｜Suspension Culture｜Chemically Defined Medium

CHO Chemically Defined Media

① Adherent Culture

・CH100 medium

② Cloning Assay

・CH150 medium

③ Suspension Culture

・CH200 medium

・CH300 medium

・CH300AZ medium＊

・CH400 medium

・CH400AZ medium＊

＊Ready-to-use products with L-alanyl-L-glutamine

Learn more

④ Fed-Batch Culture

・Gxpress CHO Feed medium

⑤ Anti-Clumping Reagent

・Scattering reagent CHO

Suspension Culture (Expression & Production)
CH300 mdium (CD medium), liquid or powder
For CHO cells "CHO-K1, CHO-S, DG44, DXB11, etc."

CHO cells｜Suspension culture｜Chemically defined & Serum-free medium

　CH300 medium is a chemically defined medium / serum-free medium optimized for the high production of recombinant proteins in Chinese hamster ovary (CHO) cells, such as CHO-K1, CHO-S, DG44, and DXB11 cells. The medium contains no proteins, hydrolysates, or human/animal-derived components. All components have a known chemical structure.

CH300 medium has the advantages of lot-to-lot consistency and the ease of downstream purification after expression of recombinant proteins. The medium supports small- and large-scale protein expression in stably or transiently transfected CHO cells.

Protocol

・CH300 medium protocol

Figure 1) Growth assay of CHO-K1 cells with CH300/CH300AZ medium.

Suspension CHO-K1 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
CH300/CH300AZ medium leads to the high performance similar to Thermo CD OptiCHO medium in cell growth for CHO-K1 cells.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Culture volume：30 mL with L-alanyl-L-glutamine.

Figure 2) Growth assay of CHO-S cells with CH300/CH300AZ medium.

Suspension CHO-S cells were cultured with disposable Erlenmeyer flasks under the following conditions.
CH300/CH300AZ medium leads to the high performance similar to Thermo CD OptiCHO medium in cell growth for CHO-S cells.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 8% CO2.
(3) Culture volume：30 mL with L-alanyl-L-glutamine.

Figure 3) Transient transfection assay of CHO-K1 cells with CH300/CH300AZ medium.

CH300/CH300AZ medium exhibits the higher performance in human IgG production compared to other company medium without inhibiting the transfection assay.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e6 cells/mL, >95% viability.
(2) Static culture：37℃, 5% CO2.

Figure 4) Transient transfection assay of CHO cells with CH300/CH300AZ medium.

CH300/CH300AZ medium exhibits the equal or higher performance in gene expression compared to other campany medium without inhibiting the transfection assay.
＜ Culture conditions ＞
(1) Seeding cell density：6 x 10e6 cells/mL, >95% viability.
(2) Shaker culture：125 rpm, 37℃, 5% CO2.
(3) Transfection reagent：Gxpress CHO TF Reagent (GMEP Inc.).

Figure 5）Growth rate of CHO-DG44 cells with CH300/CH300AZ medium.

Suspension CHO-DG44 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
CH300/CH300AZ medium leads to good cell growth for CHO-DG44 cells.
＜ Culture conditions ＞
(1) Seeding cell density：2 x 10e5 cells/mL.
(2) Shaker culture：125 rpm, 37℃, 5% CO2.
(3) Culture volume：30 mL with L-glutamine.