HOME | CH300AZ medium|CHO cells|Suspension Culture|Chemically Defined Medium

CHO Chemically Defined Media

① Adherent Culture
 
② Cloning Assay
③ Suspension Culture
*Ready-to-use products with L-alanyl-L-glutamine
④ Fed-Batch Culture
 
⑤ Anti-Clumping Reagent

Suspension Culture (Expression & Production)
CH300AZ mdium (CD medium), liquid or powder
For CHO cells "CHO-K1, CHO-S, DG44, DXB11, etc."
*A ready-to-use product that contains L-alanyl-L-glutamine.

CHO cells|Suspension culture|Chemically defined & Serum-free medium

 CH300AZ medium is a chemically defined medium / serum-free medium optimized for the high production of recombinant proteins in Chinese hamster ovary (CHO) cells, such as CHO-K1, CHO-S, DG44, and DXB11 cells. The medium contains no proteins, hydrolysates, or components of animal and plant composition. All components have a known chemical structure.

   CH300AZ medium has the advantages of lot-to-lot consistency and the ease of downstream purification after expression of recombinant proteins. The medium supports small- and large-scale protein expression in stably or transiently transfected CHO cells.

Figure 1) Growth assay of CHO-K1 cells with CH300/CH300AZ medium.

Suspension CHO-K1 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
CH300/CH300AZ medium leads to the high performance similar to Thermo CD OptiCHO medium in cell growth for CHO-K1 cells.
< Culture conditions >
(1) Seeding cell density:2 x 10e5 cells/mL.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Culture volume:30 mL with L-alanyl-L-glutamine.

Figure 2) Growth assay of CHO-S cells with CH300/CH300AZ medium.

Suspension CHO-S cells were cultured with disposable Erlenmeyer flasks under the following conditions.
CH300/CH300AZ medium leads to the high performance similar to Thermo CD OptiCHO medium in cell growth for CHO-S cells.
< Culture conditions >
(1) Seeding cell density:2 x 10e5 cells/mL.
(2) Shaker culture:125 rpm, 37℃, 8% CO2.
(3) Culture volume:30 mL with L-alanyl-L-glutamine.

Figure 3) Transient transfection assay of CHO-K1 cells with CH300/CH300AZ medium.

CH300/CH300AZ medium exhibits the higher performance in human IgG production compared to other company medium without inhibiting the transfection assay.
< Culture conditions >
(1) Seeding cell density:2 x 10e6 cells/mL, >95% viability.
(2) Static culture:37℃, 5% CO2.


Figure 4) Transient transfection assay of CHO cells with CH300/CH300AZ medium.

CH300/CH300AZ medium exhibits the equal or higher performance in gene expression compared to other campany medium without inhibiting the transfection assay.
< Culture conditions >
(1) Seeding cell density:6 x 10e6 cells/mL, >95% viability.
(2) Shaker culture:125 rpm, 37℃, 5% CO2.
(3) Transfection reagent:Gxpress CHO TF Reagent (GMEP Inc.).


Figure 5)Growth rate of CHO-DG44 cells with CH300/CH300AZ medium.

Suspension CHO-DG44 cells were cultured with disposable Erlenmeyer flasks under the following conditions.
CH300/CH300AZ medium leads to good cell growth for CHO-DG44 cells.
< Culture conditions >
(1) Seeding cell density:2 x 10e5 cells/mL.
(2) Shaker culture:125 rpm, 37℃, 5% CO2.
(3) Culture volume:30 mL with L-glutamine.


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